Endogenous hyaluronate-cell surface interactions in 3T3 and simian virus-transformed 3T3 cells.
نویسندگان
چکیده
منابع مشابه
Physical characteristics of hyaluronate binding to the surface of simian virus 40-transformed 3T3 cells.
The binding of labeled hyaluronate to the surface of Simian virus 40-transformed 3T3 cells was studied as a function of 1) pH, 2) ionic strength, 3) temperature, and 4) molecular weight of the hyaluronate. Binding occurred over a wide range of pH values with optima at pH 7 and at less than pH 4. Binding at low pH was eliminated at high ionic strength whereas that at physiological pH was enhance...
متن کاملInteraction of hyaluronate with the surface of simian virus 40-transformed 3T3 cells: aggregation and binding studies.
Previous studies have shown that the divalent-cation independent aggregation of simian virus 40-transformed 3T3 cells (SV-3T3) is mediated by the interaction of endogenous hyaluronate with binding sites on the cell surface. In the present study, the nature of the interaction of hyaluronate with the binding sites was characterized further by examining both the aggregation of SV-3T3 cells and the...
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Identification and characterization of hormone receptors on the cell surface is an effective tool for studying the plasma membrane. Using the direct binding of a radiolabeled antagonist, (-)[3H]alprenolol, to crude membrane preparations, and a physiological response (cellular cyclic AMP levels), I demonstrated a catecholamine (beta-adrenergic) hormone receptor site coupled to a catecholamine re...
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values in all of the experimental treatments. Greatest discrimination against 86Rb+ is observed at low external K+ concentrations; at higher external K+ concentrations 86Rb+ and ‘*K+ flux rates show less divergence. Table I suggests that different transport systems within the same organism may discriminate to a greater or smaller extent against 86Rb+. Somewhat similar proposals were made by Rho...
متن کاملMevalonic acid products as mediators of cell proliferation in simian virus 40-transformed 3T3 cells.
Effects of treatment with serum-free medium and 25-hydroxycholesterol (25-OH) on the cell cycle of simian virus 40-transformed 3T3 fibroblasts, designated SV-3T3 cells, were studied and compared with simultaneous effects on the activity of 3-hydroxy-3-methylglutaryl (HMG) CoA reductase and incorporation of [3H]mevalonic acid into cholesterol, Coenzyme Q, and dolichol. The data confirm our previ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1984
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)42720-7